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Please contact Michael Calderwood with questions regarding interactome projects.
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Please contact Tong Hao with your questions or comments.
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CCSB interactome mapping and ORFeome cloning efforts are supported by federal grants from the National Human Genome Research Institute, the National Cancer Institute and the National Science Foundation, and by funding from the Dana-Farber Cancer Institute Strategic Initiative, The Ellison Foundation and the W. M. Keck Foundation.

CCSB Resources

Human ORFeome v5.1

Human ORFeome v5.1 contains 15,483 human ORFs, corresponding to 12,794 human genes. This set of ORFs ranges in size from 75 to more than 10Kb base pairs, and (excluding the RT-PCR rescued ORFs) contains 1,502 genes with multiple splice variants and 814 polymorphic genes.

C. elegans ORFeome

In addition to the human draft sequence, the complete genome sequences of an increasing number of model organisms are available. (E. coli and a large number of microorganisms, S. cerevisiae, C. elegans, D. melanogaster and A. thaliana). This sequence information is expected to revolutionize the way biological questions can be addressed. Molecular mechanisms should now be approachable on a more global scale in the context of (nearly) complete sets of genes, rather than by analyzing genes individually.

Worm Interactome

Worm Interactome version 8 assembles high-quality yeast two-hybrid protein-protein interactions in C. elegans. It contains 4,189 known binary protein-protein interactions from three sources:
WI-2007, a high-throughput yeast two-hybrid screen done in the Vidal lab in 2007 (1,816 interactions)
WI-2004, a high-throughput yeast two-hybrid screen done in the Vidal lab in 2004, updated and reprocessed (1,735 interactions)
A compendium of medium-throughput biological processes based yeast two-hybrid screens updated and reprocessed (554 interactions).

Worm Localizome

In C. elegans, promoter::GFP approaches can be used to characterize expression at the single-cell resolution level; however this kind of analysis is extremely time consuming and is therefore very difficult to adapt to genome-scale analyses. This project generated a new view of the promoter activity throughout post-embryonic development in a digitized format.

Other Resources

Wormbase (current version)
The British Columbia C. elegans Gene Expression Consortium

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