Interactome Projects at CCSB

Yeast interactome version 1 (CCSB-YI1) contains high quality yeast two-hybrid protein-protein interactions in S. cerevisiae. It includes 3,151 interactions among 2,108 proteins (35% of the yeast genome) from three independent sources: CCSB-YI1, Ito-Core, and Uetz-Screen. more

Worm Interactome version 8 contains 3,864 binary protein-protein interactions assembled from the WI-2007 high throughput yeast two hybrid screen (1816 new interactions); the WI-2004 high throughput yeast two hybrid screen (1735 interactions first reported in Li, et al Science 2004); and a compendium of data from medium throughput yeast two hybrid screens (554 interactions). more

Human Interactome Mapping Project is the flagship project of CCSB. A first draft map of "Space-I" of the human interactome, that describes interactions within the matrix of 8000x8000 ORFs contained in Human ORFeome v1.1 was published in 2005 (Rual et al.). We have used these data to develop a conceptual framework that incorporates screen-completeness, assay detection-ability and screen saturation to estimate the size of the human interactome (Venkatesan et al, submitted). Currently, work is in progress to map space II (12kx12k) and III (16k x 16k) of the human interactome at three-fold coverage.more

PGRP Plant Interactome Project pertains to the development of a large-scale, high coverage binary protein-protein interaction or "interactome" map for plants that should represent a starting point for systems-level studies in plants. The recent completion of whole genome sequences for several plant species has revealed an enormous amount of conservation among their encoded proteins. more

Chlamydomonas reinhardtii is a promising "bio-energy" organism capable of producing hydrogen gas and other "bio-fuel" resources. In this Department of Energy GTL funded project, we are: i) experimentally verifying and defining the transcript structures of ~2,000 metabolically related genes and cloning their open reading frames (ORF); ii) identifying protein-protein interactions among the metabolic gene products; and iii) building protein interaction maps and metabolic networks that will ultimately allow the development of testable predictions of C. reinhardtii physiology, including gene lethality and rates of growth under defined environmental conditions. more