Yeast Interactome Project
W e carried out a comparative quality assessment of current yeast interactome datasets, demonstrating that high-throughput yeast two-hybrid (Y2H) provides high-quality binary interaction information. As a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically-controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H dataset covering ~20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high-quality but of a fundamentally different and complementary nature resulting in networks with different topological and biological properties. This binary map is enriched for transient signaling interactions and inter-complex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.
"High-Quality Binary Protein Interaction Map of the Yeast Interactome Network" Yu et al Science 2008.