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Yeast Interactome Project
W e carried out a comparative quality assessment of current yeast interactome datasets, demonstrating that high-throughput yeast two-hybrid (Y2H) provides high-quality binary interaction information. As a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically-controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H dataset covering ~20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high-quality but of a fundamentally different and complementary nature resulting in networks with different topological and biological properties. This binary map is enriched for transient signaling interactions and inter-complex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.
"High-Quality Binary Protein Interaction Map of the Yeast Interactome Network" Yu et al Science 2008.
Graphical representation of three different types of yeast interactome datasets. The structure of the binary interactome network is obviously different from the structure of the co-complex interactome network. The network structure of the literature-curated dataset resembles that of the co-complex dataset, even though the literature-curated datasets are reported to contain mostly binary interactions. All datasets can be downloaded here. |  |