Human Interactome Project

We are systematically mapping the human binary interactome with the goal of generating and analyzing high throughput yeast two-hybrid interactions with high quality and high sensitivity for all pair wise combinations of predicted gene products for which we have at least one Gateway-cloned ORF available (Human ORFeome website). This page contains data corresponding to the following sources:

(1) Our initial efforts at mapping the human interactome (Rual et al, Nature 2005). This involved screening a space (Space-I) of 8107 ORFs corresponding to 7194 genes, resulting in the mapping of 2,754 high quality Y2H interactions. This data set increased the number of binary interactions by nearly 70% over those curated in the various databases and is freely available to the research community.

(2) Our recent efforts at estimating the coverage and size of the human interactome, which involved performing four repeat Y2H screens of 1,822 DB-X fusion proteins (or "baits", representing 1,744 unique genes) against 1,796 AD-Y proteins (or "preys", representing 1,752 genes) corresponding to a 5% subset of Space-I. This resulted in the mapping of 239 high quality Y2H interactions, out of which 107 interactions are found in a single screen and 132 interactions in multiple screens ( 63 found in two screens, 44 found in three screens and 25 found in four screens).

We also have in progress a new phase of the human interactome project corresponding to an expanded search space (Space-II) reflecting a matrix of ~16,000 X 16,000 proteins, which represents ~50% of the complete search space assuming a total of ~22,000 protein-coding genes and limiting the scope to one splice variant per gene. Data from these screens will be available once the ongoing high-throughput experiments and data processing are completed.